Fig 1: The major BH4-synthesizing enzyme, Gch1 is down-regulated by BDKRB2 in Tg-B2++ApoE–/– mice. (A) Aortic gene expression level of Gch1 was quantified of indicated study groups (mean ± s.d.; n = 4; *p < 0.05 vs. B6; **p < 0.01; ***p < 0.001 vs. B6 and B2–/–ApoE–/–; other comparisons are indicated; Tukey‘s test). (B) The aortic protein content of Gch1 was determined by immunoblot with Gch1-specific antibodies. The left panel shows immunoblot detection of aortic Gch1 of indicated study groups, and the right panel shows quantitative data (mean ± s.d.; n = 6; *p < 0.05; ***p < 0.001 vs. ApoE–/– and B2–/–ApoE–/–; other comparisons are indicated; Tukey‘s test).